Lecture 13 learning objec7ves
1. Explain the difference between KA and KD .
2. Explain why we use a dissociate:on constant to describe an associate:on reac:on.
3. Illustrate the relationship between affinity and the free energy of binding.
4. Summarize the relationship between frac:onal occupancy and KD .
5. Outline classical trans formations of binding data (i.e. Scat chard analysis), and
why they are useful.
6. Draw the universal binding isotherm, and use it to relate ligand occupancy to ligand concentration:on (i.e. rela:ve to KD ).
