1. Explain the difference between affinity and specificity.
2. Illustrate the relationship between affinity and specificity.
3. Explain what the “specificity factor” (α) is.
4. Make use of various equation, solving for the “specificity factor”.
5. Summarize the relationship between specificity and free ligand concentraon
for a simple system with one on-‐target receptor and one off-‐target receptor.
6. Summarize the structural origins of affinity and specificity.
7. Define a binding “hot spot”
