1. Explain how enzymes stabilize the transi8on state of a chemical reac8on.
2. Explain mechanisms, other than transi8on state stabiliza8on, that enzymes
use to accelerate a chemical reac8on.
3. Illustrate the mechanism by which serine proteases catalyze pep8de bond cleavage, and explain how this remarkable catalysis is achieved in rela8on to the reac8on in water.
4. Describe the structural basis of substrate specificity in the family of serine proteases.
