heat Germ DNA Extraction Laboratory Report
1. In the space provide below, draw a four nucleotide piece (e.g. ATGC) of DNA,
drawing out the FULL structure showing the four different nitrogenous bases that occur
in DNA and showing it in its ionization state at physiological pH.
2. Describe the difference between the genomic DNA you isolated in this experiment and “plasmid” DNA used in recombinant DNA technology.
4. What was the weight of the DNA you isolated?
You started with 2g of raw wheat germ. Using this knowledge calculate the apparent %
of your raw wheat germ that is supposedly DNA. Show your work below.
Apparent % DNA in wheat germ:
Calculate the total amount of DNA in your isolated wheat germ DNA sample based upon
your measured A260 . Take into account any dilutions you may have made. Show your
calculations below.
Total amount of DNA in wheat germ (based on A260 ):
% DNA in wheat germ (based on A260 ):
6. Which method of measuring the amount of DNA in your wheat germ gives the larger amount of DNA?:
7. Which method do you think is a more accurate way of measuring the amount of DNA
in a sample?: Why
