Animal Subjects
If using animal subjects, you should, in lieu of a Participants section, have a separate subsection titled Animal Subjects in which you address the following five points (using a numbered outline is okay but not required).
Identify the species, strains, ages, sex, and numbers of animals to be used in the proposed work.
Justify the use of animals, the choice of species, and the numbers to be used. If animals are in short supply, costly, or to be used in large numbers, provide an additional rationale for their selection and numbers.
Provide information on the veterinary care of the animals involved.
Describe the procedures for ensuring that discomfort, distress, pain, and injury will be limited to that which is unavoidable in the conduct of scientifically sound research. Describe the use of analgesic, anesthetic, and tranquilizing drugs and/or comfortable restraining devices, where appropriate, to minimize discomfort, distress, pain, and injury.
Describe any method of euthanasia to be used and the reasons for its selection. State whether this method is consistent with the recommendations of the Panel on Euthanasia of the American Veterinary Medical Association. If not, present a justification for not following the recommendations. If the method was used by previous researchers, you may assume it is consistent with those recommendations.
